Chromatography of Erythrocyte Sialoglycopeptides on DEAE-Cellulose Paper and DEAE-Sephadex Thin-Layer Plate
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چکیده
منابع مشابه
Electrophoresis on cellulose acetate and chromatography on DEAE-sephadex A-50 compared in the estimation of creatine kinase isoenzymes.
We have compared two methods for separating the isoenzymes of creatine kinase in serum and measuring their activity: chromatography on diethylaminoethyl-Sephadex A-50, with both continuous and discontinuous gradient elution, and electrophoresis on cellulose acetate. Results by continuous and discontinuous gradient elution correlated well both for tissue extracts and sera. Electrophoresis on cel...
متن کاملPurification of peptidyl-tRNA on benzoylated DEAE-cellulose columns.
The o-nitrophenylsulfenyl group, and amino protecting group in the chemical synthesis of peptidyl-tRNA was used to attach newly synthesized peptidyl-tRNA to a benzoylated DEAE-cellulose column. This facilitated the isolation of a highly purified specific tRNA with a well defined peptide chain.
متن کاملACE was solubilized from human kidney cortex with trypsin and purified to homogeneity [41. Purification procedures included fractionation with DEAE-cellulose, hydroxyapatite and DEAE-Sephadex A-50, preparative isoeletric focusing and gel filtration on Sephadex
1. Human angiotensin-converting enzyme from kidney, lung, small intestine, plasma and urine was found to be immunologically indistinguishable by double immunodiffusion and inhibition of enzyme activity with specific anti(human kidney enzyme) antibody. 2. Partial inhibition of the enzyme activities from hog, dog and rat sera by the antibody was found, but no inhibition was observed in the enzyme...
متن کاملThe Lipophilicity Examination of Some ACE inhibitors and Hydrochlorothiazide on Cellulose in RP Thin-Layer Chromatography
In this assay, the evaluation of lipophilicity of four ACE-inhibitors and hydrochlorothiazide (HCTZ) with RP-TLC on cellulose layers was described using three binary solvent systems. The selected ACE inhibitors had sufficiently different structures which can indicate the method suitability for their lipophilicity evaluation as the model substances in comparison with HCTZ. In addition, the linea...
متن کاملThe Lipophilicity Examination of Some ACE inhibitors and Hydrochlorothiazide on Cellulose in RP Thin-Layer Chromatography
In this assay, the evaluation of lipophilicity of four ACE-inhibitors and hydrochlorothiazide (HCTZ) with RP-TLC on cellulose layers was described using three binary solvent systems. The selected ACE inhibitors had sufficiently different structures which can indicate the method suitability for their lipophilicity evaluation as the model substances in comparison with HCTZ. In addition, the linea...
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ژورنال
عنوان ژورنال: Proceedings of the Japan Academy
سال: 1967
ISSN: 0021-4280
DOI: 10.2183/pjab1945.43.674